1 liter agar how many plates

Decide how many plates you will need. Our recipes will make 1 L mL of media, enough to fill approximately forty mm plates, but they can be scaled up or down as needed. Plan on using about 25 mL per mm plate. Use a glass container ideally an Erlenmeyer flask that will hold at least twice the volume of your media.

Make sure the agar dissolves completely. The media may look cloudy, or you may see small, translucent lens-like objects floating in it. Continue boiling until the media is completely clear; this may take longer than 15 minutes. Incompletely dissolved agar will leave your media squishy or fragile.

Log in Register. Log in. Create an Account First Name:. Last Name:. Confirm Password. I am a teacher. Sign me up for the newsletter. Why should I sign up? Reset Password Email:. Tip: Microbiologists typically use sterile, disposable polystyrene plates, but you can also use glass Petri dishes or even baby food jars. Now you should have no issues making agar plates that are perfect every time.

Originally published July 5, Reviewed and updated February Has this helped you? Then please share with your network. Hi pouring in plates should be under laminar hood? I have to autoclave the plates before pouring?

Hi, I made NGM agar with the powder and water, added the dextrose, autoclaved it, then plated it. I found out today that I missed out on 3 ingredients. Can I save the current media by heating the plates and returning the liquid to the jar I made it in, and add the required ingredients and autoclave again?

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What is the exact amount of agar that should be added in ml of media? Catherine E Davis-Sparks on September 27, at am. Privacy Policy. Password recovery. Top Tip Bio. Measure out approximately mL of distilled water and add to the Duran bottle.

Shake the bottle to dissolve the reagents. Once the reagents have fully dissolved, adjust the pH to 7. Once the pH is adjusted, top up the solution to mL by using distilled water. Optional: set a water bath to 55 o C. To sterilise, autoclave the solution on a liquid cycle 20 min at 15 psi. Leave the solution to cool to approximately 55 o C, or warm enough to be held in your hand without burning it.

If you do not have enough time to keep an eye on the temperature, put the bottle into the water bath.